Biotechnology: Principles and Processes

The correct order of steps in Polymerase Chain Reaction (PCR) is

1. extension, denaturation, annealing
2. annealing, extension, denaturation
3. denaturation, extension, annealing
4. denaturation, annealing, extension

The DNA fragments separated on an agarose gel can be visualised after staining with

1. Bromophenol blue
2. Aniline blue
3. Ethidium bromide
4. Acetocarmine

DNA fragments are

1. Positively charged
2. Either positively or negatively charged depending on their size
3. Neutral
4. Negatively charged

A gene whose expression helps to identify transformed cell is known as

1. Selectable marker
2. Plasmid
3. Vector
4. Structural gene

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

1. Positively charged fragments move to farther end
2. Negatively charged fragments do not move
3. The smaller the fragment size, the farther it moves
4. The larger the fragment size, the farther it moves

The process of separation and purification of expressed protein before marketing is called :

1. Upstream processing
2. Bioprocessing
3. Downstream processing
4. Postproduction processing

Which of the following is not a feature of the plasmids ?

1. Single-stranded
2. Transferable
3. Independent replication
4. Circular structure

The taq polymerase enzyme is obtained from

1. Thermus aquaticus
2. Bacillus subtilis
3. Pseudomonas putida
4. Thiobacillus ferrooxidans

Which of the following is a restriction endonuclease ?

1. DNase I
2. Hind II
3. RNase I
4. Protease

Stirred-tank bioreactors have been designed for -

1. ensuring anaerobic conditions in the culture vessel
2. addition of preservatives to the product
3. purification of product
4. availability of oxygen throughout the process