Biotechnology: Principles and Processes

A gene whose expression helps to identify transformed cell is known as

1. Selectable marker
2. Plasmid
3. Vector
4. Structural gene

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

1. Positively charged fragments move to farther end
2. Negatively charged fragments do not move
3. The smaller the fragment size, the farther it moves
4. The larger the fragment size, the farther it moves

The process of separation and purification of expressed protein before marketing is called :

1. Upstream processing
2. Bioprocessing
3. Downstream processing
4. Postproduction processing

Which of the following is not a feature of the plasmids ?

1. Single-stranded
2. Transferable
3. Independent replication
4. Circular structure

The taq polymerase enzyme is obtained from

1. Thermus aquaticus
2. Bacillus subtilis
3. Pseudomonas putida
4. Thiobacillus ferrooxidans

Which of the following is a restriction endonuclease ?

1. DNase I
2. Hind II
3. RNase I
4. Protease

Stirred-tank bioreactors have been designed for -

1. ensuring anaerobic conditions in the culture vessel
2. addition of preservatives to the product
3. purification of product
4. availability of oxygen throughout the process

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

1. Eco RI
2. Polymerase III
3. Ligase
4. Taq polymerase

Which of the following is not a component of downstream processing ?

1. Purification
2. Expression
3. Preservation
4. Separation

Which of the following restriction enzymes produces blunt ends ?

1. Eco RV
2. Hind III
3. Xho I
4. Sal I