Biotechnology: Principles and Processes

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

1. Eco RI
2. Polymerase III
3. Ligase
4. Taq polymerase

Which of the following is not a component of downstream processing ?

1. Purification
2. Expression
3. Preservation
4. Separation

Which of the following restriction enzymes produces blunt ends ?

1. Eco RV
2. Hind III
3. Xho I
4. Sal I

The DNA molecule to which the gene of interest is integrated for cloning is called -

1. Transformer
2. Template
3. Vector
4. Carrier

The cutting of DNA at specific locations became possible with the discovery of -

1. Restriction enzymes
2. Selectable markers
3. Probes
4. Ligases

The introduction of T-DNA into plants involves -

1. Altering the pH of the soil, then heat-shocking the plants
2. Infection of the plant by Agrobacterium tumefaciens
3. Exposing the plants to cold for a brief period
4. Allowing the plant roots to stand in water

Which vector can clone only a small fragment of DNA ?

1. Cosmid
2. Bacterial artificial chromosome
3. Yeast artificial chromosome
4. Plasmid

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of :

1. Insertional inactivation of alphagalactosidase in recombinant bacteria
2. Inactivation of glycosidase enzyme in recombinant bacteria
3. Insertional inactivation of alphagalactosidase in non-recombinant bacteria
4. Non-recombinant bacteria containing betagalactosidase

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

1. Polymerase chain reaction
2. Restriction mapping
3. Electrophoresis
4. Centrifugation

Which of the following is not correctly matched for the organism and its cell wall degrading enzyme ?

1. Plant cells- Cellulase
2. Algae-Methylase
3. Fungi - Chitinase
4. Bacteria-Lysozyme