Biotechnology: Principles and Processes

Which of the following is not a feature of the plasmids ?

1. Single-stranded
2. Transferable
3. Independent replication
4. Circular structure

The taq polymerase enzyme is obtained from

1. Thermus aquaticus
2. Bacillus subtilis
3. Pseudomonas putida
4. Thiobacillus ferrooxidans

Which of the following is a restriction endonuclease ?

1. DNase I
2. Hind II
3. RNase I
4. Protease

Stirred-tank bioreactors have been designed for -

1. ensuring anaerobic conditions in the culture vessel
2. addition of preservatives to the product
3. purification of product
4. availability of oxygen throughout the process

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

1. Eco RI
2. Polymerase III
3. Ligase
4. Taq polymerase

Which of the following is not a component of downstream processing ?

1. Purification
2. Expression
3. Preservation
4. Separation

Which of the following restriction enzymes produces blunt ends ?

1. Eco RV
2. Hind III
3. Xho I
4. Sal I

The DNA molecule to which the gene of interest is integrated for cloning is called -

1. Transformer
2. Template
3. Vector
4. Carrier

The cutting of DNA at specific locations became possible with the discovery of -

1. Restriction enzymes
2. Selectable markers
3. Probes
4. Ligases

The introduction of T-DNA into plants involves -

1. Altering the pH of the soil, then heat-shocking the plants
2. Infection of the plant by Agrobacterium tumefaciens
3. Exposing the plants to cold for a brief period
4. Allowing the plant roots to stand in water