Biotechnology and its Applications

Match List I with List II :

1. A-II, B-I, C-IV, D-III
2. A-II, B-III, C-I, D-IV
3. A-I, B-IV, C-III, D-II
4. A-I, B-II, C-IV, D-III

Arrange the following steps of somatic hybridisation in a correct sequence. Choose the correct answer from the options given below :

1. D, B, A, E, C
2. E, A, B, C, D
3. E, B, A, D, C
4. D, A, B, C, E

The toxin proteins isolated from Bacillus thuringiensis, coded by which of the following genes would control cotton bollworms and corn borer, respectively?

1. cryIAc and cryIIAb
2. cryIAc and cryIIIAb
3. cryIAc and cryIAb
4. cryIIAb and cryIAc

The inactive form of Bt toxin is converted to the active form in the insect gut

1. by nucleases
2. due to alkaline pH
3. due to acidic pH
4. by proteases

Silencing of specific mRNA is possible via RNAi because of

1. Complementary tRNA
2. Non-complementary ssRNA
3. Complementary dsRNA
4. Inhibitory ssRNA

Given below are two statements: In the light of the above statements, choose the correct answer from the options given below:

1. Both Statement I and Statement II are true
2. Both Statement I and Statement II are false
3. Statement I is true but Statement II is false
4. Statement I is false but Statement II is true

Match List I with List II Choose the correct answer from the options given below:

1. A-II, B-I, C-IV, D-III
2. A-III, B-I, C-II, D-IV
3. A-III, B-IV, C-I, D-II
4. A-II, B-IV, C-I, D-III

The Bt toxin in genetically engineered Bt cotton kills the pest by:

1. Creating pores in the midgut
2. Damaging the respiratory system
3. Degenerating the nervous system
4. Altering the pH of body fluids

Following are the steps involved in action of toxin in Bt. Cotton Choose the correct sequence of steps from the options given below:

1. E C B A D
2. B C A E D
3. A E B D C
4. B E C A D

Given below are two statements : In the light of the above statements, choose the correct answer from the options given below.

1. Statement I is true but Statement II is false.
2. Statement I is false but Statement II is true.
3. Both Statement I and Statement II are true.
4. Both Statement I and Statement II are false.

Transposons can be used during which one of the following?

1. Polymerase Chain Reaction
2. Gene Silencing
3. Autoradiography
4. Gene sequencing

Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to

1. Insect predators
2. Insect pests
3. Fungal diseases
4. Plant nematodes

Match the following columns and select the correct option.

1. (a)-(i), (b)-(ii), (c)-(iii), (d)-(iv)
2. (a)-(iii), (b)-(ii), (c)-(i), (d)-(iv)
3. (a)-(iv), (b)-(i), (c)-(ii), (d)-(iii)
4. (a)-(ii), (b)-(iii), (c)-(iv), (d)-(i)

What triggers activation of protoxin to active Bt toxin of Bacillus thuringiensis in boll worm?

1. Acidic pH of stomach
2. Body temperature
3. Alkaline pH of gut
4. Moist surface of midgut

Which of the following is true for Golden rice?

1. It is drought tolerant, developed using Agrobacterium vector.
2. It has yellow grains, because of a gene introduced from a primitive variety of rice.
3. It is Vitamin A enriched, with a gene from daffodil.
4. It is pest resistant, with a gene from Bacillus thuringiensis.

Which part of the tobacco plant is infected by Meloidogyne incognita ?

1. Flower
2. Stem
3. Leaf
4. Root

Golden rice is a genetically modified crop plant where the incorporated gene is meant for biosynthesis of -

1. Vitamin C
2. Omega 3
3. Vitamin A
4. Vitamin B

In Bt cotton, the Bt toxin present in plant tissue as pro-toxin is converted into active toxin due to :

1. action of gut micro-organisms
2. presence of conversion factors in insect gut
3. alkaline pH of the insect gut
4. acidic pH of the insect gut

The crops engineered for glyphosate are resistant/tolerant to :

1. Bacteria
2. Herbicides
3. Fungi
4. Insects

Which of the following Bt crops is being grown in India by the farmers ?

1. Soybean
2. Cotton
3. Brinjal
4. Maize

RNA interference involves :-

1. Interference of RNA in synthesis of DNA
2. Synthesis of cDNA from RNA using reverse transcriptase
3. Synthesis of mRNA from DNA
4. Silencing of specific mRNA due to complementary RNA

Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells) :

1. An antifeedant
2. A toxic protein
3. Both sense and anti-sense RNA
4. A particular hormone

Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin 'A' deficiency?

1. Flavr Savr tomato
2. Canola
3. Bt-brinjal
4. Golden rice

Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein:

1. is activated by acid pH of the foregut of the insect pest.
2. binds with epithelial cells of midgut of the insect pest ultimately killing it
3. is coded by several genes including the gene cry
4. does not kill the carrier bacterium which is itself resistant to this toxin

Silencing of mRNA has been used in producing transgenic plants resistant to:

1. White rusts
2. Nematodes
3. Bacterial blights
4. Bollworms

The process of RNA interference has been used in the development of plants resistant to :

1. Nematodes
2. Fungi
3. Viruses
4. Insects

The genetically-modified (GM) brinjal in India has been developed for:

1. Enhancing shelf life
2. Insect-resistance
3. Drought-resistance
4. Enhancing mineral content

Some of the characteristics of Bt cotton are –

1. High yield and production of toxic protein crystals which kill dipteran pests
2. High yield and resistance to bollworms
3. Long fibre and resistance to aphids
4. Medium yield, long fibre and resistance to beetle pests

An improved variety of transgenic basmati rice

1. gives high yield but has no characteristic aroma
2. does not require chemical fertilizers and growth hormones
3. is completely resistant to all insect pests and diseases of paddy
4. gives high yield and is rich in vitamin A

What is true about Bt toxin?

1. The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication.
2. Bt protein exists as active toxin in the Bacillus
3. The concerned Bacillus has antitoxins
4. The inactive protoxin gets converted into active form in the insect gut