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Biotechnology and its Applications

AIPMT 2012 (Mains)MCQEasy📈 Topic in 75% papers+4−1
Ques. 1AIPMT 2012 (Mains)

Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells) :

  1. An antifeedant
  2. A toxic protein
  3. Both sense and anti-sense RNA
  4. A particular hormone

Answer

(3) Both sense and anti-sense RNA

Solution

As per NCERT, a nematode Meloidogyne incognita infects the roots of tobacco plants; resistance was obtained by RNA interference (RNAi).

⇒ Using Agrobacterium vectors, nematode-specific genes were introduced so that they produced both sense and anti-sense RNA in the host cells.

∴ These complementary RNAs formed dsRNA, which silenced the specific mRNA of the nematode, and the parasite could not survive.

Answer: (3)

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Ques. 2AIPMT 2012 (Mains)

The first clinical gene therapy was given for treating

  1. Rheumatoid arthritis
  2. Adenosine deaminase deficiency
  3. Diabetes mellitus
  4. Chicken pox
Ques. 3AIPMT 2012 (Prelims)

A single strand of nucleic acid tagged with a radioactive molecule is called

  1. Probe
  2. Selectable marker
  3. Vector
  4. Plasmid
Ques. 4AIPMT 2012 (Prelims)

Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin 'A' deficiency?

  1. Flavr Savr tomato
  2. Canola
  3. Bt-brinjal
  4. Golden rice
Ques. 5AIPMT 2011 (Mains)

Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein:

  1. is activated by acid pH of the foregut of the insect pest.
  2. binds with epithelial cells of midgut of the insect pest ultimately killing it
  3. is coded by several genes including the gene cry
  4. does not kill the carrier bacterium which is itself resistant to this toxin
Ques. 6AIPMT 2011 (Mains)

Silencing of mRNA has been used in producing transgenic plants resistant to:

  1. White rusts
  2. Nematodes
  3. Bacterial blights
  4. Bollworms
Ques. 7AIPMT 2011 (Prelims)

Maximum number of existing transgenic animals is of :

  1. Mice
  2. Cow
  3. Pig
  4. Fish
Ques. 8AIPMT 2011 (Prelims)

The process of RNA interference has been used in the development of plants resistant to :

  1. Nematodes
  2. Fungi
  3. Viruses
  4. Insects
Ques. 9AIPMT 2010 (Mains)

Which one of the following is now being commercially produced by biotechnological procedures?

  1. Insulin
  2. Morphine
  3. Nicotine
  4. Quinine
Ques. 10AIPMT 2010 (Prelims)

DNA or RNA segment tagged with a radioactive molecule is called –

  1. Clone
  2. Probe
  3. Plasmid
  4. Vector
Ques. 11AIPMT 2010 (Prelims)

The genetically-modified (GM) brinjal in India has been developed for:

  1. Enhancing shelf life
  2. Insect-resistance
  3. Drought-resistance
  4. Enhancing mineral content

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