Biotechnology and its Applications

In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because:

1. Retroviral vector is introduced into these lymphocytes.
2. Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages
3. Lymphocytes from patient's blood are grown in culture, outside the body
4. Genetically engineered lymphocytes are not immortal cells.

Statements related to human Insulin are given below. Which statements is/are correct about genetically engineered Insulin? Choose the most appropriate answer from the options given below :

1. A, B and D only
2. B only
3. C and D only
4. C, D and E only

Milk of transgenic ‘Cow Rosie’ was nutritionally more balanced product for human babies than natural cow milk because it contained:

1. Human enzyme Adenosine Deaminase (ADA)
2. Human protein α-1-antitrypsin
3. Human alpha-lactalbumin
4. Human insulin-like growth factor

When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as :

1. Safety testing
2. Biopiracy
3. Gene therapy
4. Molecular diagnosis

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

1. Detection of gene mutation
2. Molecular diagnosis
3. Gene amplification
4. Purification of isolated protein

Now-a-days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise to its complementary DNA in a clone of cells, followed by its detection using autoradiography because:

1. mutated gene does not appear on photographic film as the probe has complementarity with it
2. mutated gene partially appears on a photographic film
3. mutated gene completely and clearly appears on a photographic film
4. mutated gene does not appear on a photographic film as the probe has no complementarity with it

With regard to insulin choose correct options. Choose the correct answer from the options given below.

1. A and D only
2. B and D only
3. B and C only
4. A, C and D only

For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?

1. Hybridization Technique
2. Western Blotting Technique
3. Southern Blotting Technique
4. ELISA Technique

The Adenosine deaminase deficiency results into

1. Addison's disease
2. Dysfunction of immune system
3. Parkinson's disease
4. Digestive disorder

Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to

1. Insect predators
2. Insect pests
3. Fungal diseases
4. Plant nematodes