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Biotechnology and its Applications

AIPMT 2009MCQEasy📈 Topic in 75% papers+4−1
Ques. 1AIPMT 2009

What is true about Bt toxin?

  1. The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication.
  2. Bt protein exists as active toxin in the Bacillus
  3. The concerned Bacillus has antitoxins
  4. The inactive protoxin gets converted into active form in the insect gut

Answer

(4) The inactive protoxin gets converted into active form in the insect gut

Solution

As per NCERT, Bacillus thuringiensis forms protein crystals containing a toxic insecticidal protein.

The Bt toxin protein exists as an inactive protoxin in the bacterium, it does not kill the Bacillus itself.

Once an insect ingests it, the alkaline pH of the gut solubilises the crystals and the inactive protoxin is converted into the active toxin.

The active toxin binds to the surface of midgut epithelial cells and creates pores that cause swelling and lysis, and finally the death of the insect.

Answer: (4)

Keep practising — Biotechnology and its Applications

Ques. 2AIPMT 2009

Transgenic plants are the ones -

  1. Produced after protoplast fusion in artificial medium
  2. Produced by a somatic embryo in artificial medium
  3. Generated by introducing foreign DNA into a cell and regenerating a plant from that cell
  4. Grown in artificial medium after hybridization in the field
Ques. 3AIPMT 2009

What is antisense technology?

  1. RNA polymerase producing DNA
  2. Production of somaclonal variants in tissue cultures
  3. When a piece of RNA that is complementary in sequence is used to stop expression of a specific gene
  4. A cell displaying a foreign antigen used for synthesis of antigens
Ques. 4AIPMT 2008

Cry I endotoxins obtained from Bacillus thuringiensis are effective against:

  1. Mosquitoes
  2. Flies
  3. Nematodes
  4. Boll worms
Ques. 5AIPMT 2008

Main objective of production/use of herbicide resistant GM crops is to:

  1. eliminate weeds from the field without the use of manual labour
  2. eliminate weeds from the field without the use of herbicides
  3. encourage eco-friendly herbicides
  4. reduce herbicide accumulation in food articles for health safety
Ques. 6AIPMT 2008

Human insulin is being commercially produced from a transgenic species of:

  1. Escherichia
  2. Mycobacterium
  3. Rhizobium
  4. Saccharomyces
Ques. 7AIPMT 2008

A transgenic food crop which may help in solving the problem of night blindness in developing countries is

  1. Flavr Savr tomatoes
  2. Bt soybean
  3. Starlink maize
  4. Golden rice
Ques. 8AIPMT 2006

Golden rice is a promising transgenic crop. When released for cultivation, it will help in

  1. Herbicide tolerance
  2. Alleviation of vitamin A deficiency
  3. Producing a petrol-like fuel from rice
  4. Pest resistance
Ques. 9AIPMT 2005

Bacillus thuringiensis (Bt) strains have been used for designing novel -

  1. Bio-metallurgical techniques
  2. Biofertilizers
  3. Bioinsecticidal plants
  4. Bio-mineralization processes
Ques. 10AIPMT 2005

Golden rice is a transgenic crop of the future with the following improved trait -

  1. High lysine (essential amino acid) content
  2. High protein content
  3. Insect resistance
  4. High vitamin A content
Ques. 11AIPMT 2004

ELISA is used to detect viruses, where the key reagent is :-

  1. Catalase
  2. RNase
  3. DNA probes
  4. Alkaline phosphatase

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