Biotechnology and its Applications

AIPMT 2004MCQMedium📈 Topic in 12% papers+4−1

Ques. 1AIPMT 2004

ELISA is used to detect viruses, where the key reagent is :-

Catalase
RNase
DNA probes
Alkaline phosphatase

Answer

(4) Alkaline phosphatase

Solution

ELISA (Enzyme Linked Immunosorbent Assay) works on the principle of antigen–antibody interaction.

The detecting (secondary) antibody is linked to an enzyme, commonly alkaline phosphatase (or peroxidase), which acts on its substrate to give a coloured product and so shows the presence of the viral antigen.

∴ The key reagent is alkaline phosphatase.

Answer: (4)

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Ques. 2AIPMT 2003

Maximum application of animal cell culture technology today is in the production of :-

Interferons
Vaccines
Edible proteins
Insulin

Ques. 3AIPMT 2003

ELISA is used to detect viruses, where the key reagent is :-

Alkaline phosphatase
RNase
DNA probes
Catalase

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