Biotechnology: Principles and Processes

Stirred-tank bioreactors have been designed for –

1. Purification of the product
2. Availability of oxygen throughout the process
3. Ensuring anaerobic conditions in the culture vessel
4. Addition of preservatives to the product

Which of the following are used in gene cloning ?

1. Mesosomes
2. Nucleoids
3. Lomasomes
4. Plasmids

Polyethylene glycol method is used for:

1. Biodiesel production
2. Seedless fruit production
3. Energy production from sewage
4. Gene transfer without a vector

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

1. Trichoderma harzianum
2. Meloidogyne incognita
3. Agrobacterium tumefaciens
4. Penicillium expansum

Gel electrophoresis is used for:

1. cutting of DNA into fragments
2. separation of DNA fragments according to their size
3. construction of recombinant DNA by joining with cloning vectors
4. isolation of DNA molecule

The linking of antibiotic resistance gene with the plasmid vector became possible with:

1. Exonucleases
2. Endonucleases
3. DNA ligase
4. DNA polymerase

A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of-

1. Trichoderma
2. Xanthomonas
3. Bacillus
4. Pseudomonas

Restriction endonuclease -

1. Cuts the DNA molecule at specific sites
2. Synthesizes DNA
3. Restricts the synthesis of DNA inside the nucleus
4. Cuts the DNA molecule randomly

Two microbes found to be very useful in genetic engineering are-

1. Diplococcus sp. and Pseudomonas sp.
2. Vibrio cholerae and a tailed bacteriophage
3. Crown gall bacterium and Caenorhabditis elegans
4. Escherichia coli and Agrobacterium tumefaciens

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?

1. Protease
2. Endonuclease
3. Lipase
4. Exonuclease