Biotechnology: Principles and Processes

Gel electrophoresis is used for:

1. cutting of DNA into fragments
2. separation of DNA fragments according to their size
3. construction of recombinant DNA by joining with cloning vectors
4. isolation of DNA molecule

The linking of antibiotic resistance gene with the plasmid vector became possible with:

1. Exonucleases
2. Endonucleases
3. DNA ligase
4. DNA polymerase

A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of-

1. Trichoderma
2. Xanthomonas
3. Bacillus
4. Pseudomonas

Restriction endonuclease -

1. Cuts the DNA molecule at specific sites
2. Synthesizes DNA
3. Restricts the synthesis of DNA inside the nucleus
4. Cuts the DNA molecule randomly

Two microbes found to be very useful in genetic engineering are-

1. Diplococcus sp. and Pseudomonas sp.
2. Vibrio cholerae and a tailed bacteriophage
3. Crown gall bacterium and Caenorhabditis elegans
4. Escherichia coli and Agrobacterium tumefaciens

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?

1. Protease
2. Endonuclease
3. Lipase
4. Exonuclease

The most thoroughly studied of the known bacteria-plant interactions is the :-

1. Nodulation of Sesbania stems by nitrogen fixing bacteria
2. Cyanobacterial symbiosis with some aquatic ferns
3. Plant growth stimulation by phosphate-solubilising bacteria
4. Gall formation on certain angiosperms by Agrobacterium

Restriction endonucleases :-

1. Are used for in vitro DNA synthesis
2. Are present in mammalian cells for degradation of DNA when the cell dies
3. Are synthesized by bacteria as part of their defense mechanism
4. Are used in genetic engineering for ligating two DNA molecules

In transgenics expression of transgene in target tissue is determined by :-

1. Enhancer
2. Promoter
3. Reporter
4. Transgene

The Ti plasmid is often used for making transgenic plants. This plasmid is found in

1. Agrobacterium
2. Rhizobium of the roots of leguminous plants
3. Azotobacter
4. Yeast as a 2 plasmid