Biotechnology: Principles and Processes

Restriction endonucleases :-

1. Are used for in vitro DNA synthesis
2. Are present in mammalian cells for degradation of DNA when the cell dies
3. Are synthesized by bacteria as part of their defense mechanism
4. Are used in genetic engineering for ligating two DNA molecules

In transgenics expression of transgene in target tissue is determined by :-

1. Enhancer
2. Promoter
3. Reporter
4. Transgene

The Ti plasmid is often used for making transgenic plants. This plasmid is found in

1. Agrobacterium
2. Rhizobium of the roots of leguminous plants
3. Azotobacter
4. Yeast as a 2 plasmid

Which one of the following bacteria has found extensive use in genetic engineering work in plants ?

1. Xanthomonas citri
2. Agrobacterium tumefaciens
3. Bacillus coagulans
4. Clostridium septicum

Which of the following enzymes are used to join bits of DNA ?

1. Endonuclease
2. DNA polymerase
3. Ligase
4. Primase

Manipulation of DNA in genetic engineering became possible due to the discovery of : -

1. Transcriptase
2. DNA ligase
3. Primase
4. Restriction endonuclease

Which of the following cuts the DNA from specific places?

1. Exonuclease
2. Alkaline phosphatase
3. E. coli restriction endonuclease I
4. Ligase

Maximum number of bases in plasmids discovered so far:

1. 500 kilobases
2. 5 kilobases
3. 50 kilobases
4. 5000 kilobases

The bacteria generally used for genetic engineering is:

1. Clostridium
2. Pseudomonas
3. Agrobacterium
4. Bacillus

Plasmid has been used as vector because:

1. Both ends show replication
2. It has antibiotic resistance gene
3. It is circular DNA which has capacity to join to eukaryotic DNA
4. It can move between prokaryotic and eukaryotic cells