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Biotechnology: Principles and Processes

AIPMT 2003MCQEasy📈 Topic in 88% papers+4−1
Ques. 1AIPMT 2003

Which one of the following bacteria has found extensive use in genetic engineering work in plants ?

  1. Xanthomonas citri
  2. Agrobacterium tumefaciens
  3. Bacillus coagulans
  4. Clostridium septicum

Answer

(2) Agrobacterium tumefaciens

Solution

As per NCERT, Agrobacterium tumefaciens, a pathogen of several dicot plants, is able to deliver a piece of DNA known as T-DNA to transform normal plant cells into a tumour.

⇒ The tumour-inducing (Ti) plasmid has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to deliver genes of interest into plants.

∴ Agrobacterium tumefaciens is extensively used in plant genetic engineering.

Answer: (2)

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Ques. 2AIPMT 2002

Which of the following enzymes are used to join bits of DNA ?

  1. Endonuclease
  2. DNA polymerase
  3. Ligase
  4. Primase
Ques. 3AIPMT 2002

Manipulation of DNA in genetic engineering became possible due to the discovery of : -

  1. Transcriptase
  2. DNA ligase
  3. Primase
  4. Restriction endonuclease
Ques. 4AIPMT 2001

Which of the following cuts the DNA from specific places?

  1. Exonuclease
  2. Alkaline phosphatase
  3. E. coli restriction endonuclease I
  4. Ligase
Ques. 5AIPMT 2001

Maximum number of bases in plasmids discovered so far:

  1. 500 kilobases
  2. 5 kilobases
  3. 50 kilobases
  4. 5000 kilobases
Ques. 6AIPMT 2000

The bacteria generally used for genetic engineering is:

  1. Clostridium
  2. Pseudomonas
  3. Agrobacterium
  4. Bacillus
Ques. 7AIPMT 2000

Plasmid has been used as vector because:

  1. Both ends show replication
  2. It has antibiotic resistance gene
  3. It is circular DNA which has capacity to join to eukaryotic DNA
  4. It can move between prokaryotic and eukaryotic cells

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