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Biotechnology: Principles and Processes

AIPMT 2002MCQEasy📈 Topic in 88% papers+4−1
Ques. 1AIPMT 2002

Which of the following enzymes are used to join bits of DNA ?

  1. Endonuclease
  2. DNA polymerase
  3. Ligase
  4. Primase

Answer

(3) Ligase

Solution

As per NCERT, DNA ligase joins DNA fragments by forming phosphodiester bonds between their ends (e.g., Okazaki fragments in replication and the vector–insert DNA in recombinant DNA technology).

Endonucleases cut DNA, DNA polymerase adds nucleotides, and primase makes RNA primers.

∴ Ligase is used to join bits of DNA ('molecular glue').

Answer: (3)

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Ques. 2AIPMT 2002

Manipulation of DNA in genetic engineering became possible due to the discovery of : -

  1. Transcriptase
  2. DNA ligase
  3. Primase
  4. Restriction endonuclease
Ques. 3AIPMT 2001

Which of the following cuts the DNA from specific places?

  1. Exonuclease
  2. Alkaline phosphatase
  3. E. coli restriction endonuclease I
  4. Ligase
Ques. 4AIPMT 2001

Maximum number of bases in plasmids discovered so far:

  1. 500 kilobases
  2. 5 kilobases
  3. 50 kilobases
  4. 5000 kilobases
Ques. 5AIPMT 2000

The bacteria generally used for genetic engineering is:

  1. Clostridium
  2. Pseudomonas
  3. Agrobacterium
  4. Bacillus
Ques. 6AIPMT 2000

Plasmid has been used as vector because:

  1. Both ends show replication
  2. It has antibiotic resistance gene
  3. It is circular DNA which has capacity to join to eukaryotic DNA
  4. It can move between prokaryotic and eukaryotic cells

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