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Biotechnology: Principles and Processes

AIPMT 2009MCQMedium📈 Topic in 50% papers+4−1
Ques. 1AIPMT 2009

Polyethylene glycol method is used for:

  1. Biodiesel production
  2. Seedless fruit production
  3. Energy production from sewage
  4. Gene transfer without a vector

Answer

(4) Gene transfer without a vector

Solution

Polyethylene glycol (PEG) makes the plasma membrane of protoplasts permeable (and causes protoplast fusion).

⇒ Naked DNA present in the medium is then taken up directly by the protoplasts.

∴ The PEG method is a direct (vectorless) gene transfer technique, like micro-injection and biolistics.

Answer: (4)

Keep practising — Biotechnology: Principles and Processes

Ques. 2AIPMT 2009

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

  1. Trichoderma harzianum
  2. Meloidogyne incognita
  3. Agrobacterium tumefaciens
  4. Penicillium expansum
Ques. 3AIPMT 2008

Gel electrophoresis is used for:

  1. cutting of DNA into fragments
  2. separation of DNA fragments according to their size
  3. construction of recombinant DNA by joining with cloning vectors
  4. isolation of DNA molecule
Ques. 4AIPMT 2008

The linking of antibiotic resistance gene with the plasmid vector became possible with:

  1. Exonucleases
  2. Endonucleases
  3. DNA ligase
  4. DNA polymerase
Ques. 5AIPMT 2007

A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of-

  1. Trichoderma
  2. Xanthomonas
  3. Bacillus
  4. Pseudomonas
Ques. 6AIPMT 2006

Restriction endonuclease -

  1. Cuts the DNA molecule at specific sites
  2. Synthesizes DNA
  3. Restricts the synthesis of DNA inside the nucleus
  4. Cuts the DNA molecule randomly
Ques. 7AIPMT 2006

Two microbes found to be very useful in genetic engineering are-

  1. Diplococcus sp. and Pseudomonas sp.
  2. Vibrio cholerae and a tailed bacteriophage
  3. Crown gall bacterium and Caenorhabditis elegans
  4. Escherichia coli and Agrobacterium tumefaciens
Ques. 8AIPMT 2005

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain?

  1. Protease
  2. Endonuclease
  3. Lipase
  4. Exonuclease
Ques. 9AIPMT 2004

The most thoroughly studied of the known bacteria-plant interactions is the :-

  1. Nodulation of Sesbania stems by nitrogen fixing bacteria
  2. Cyanobacterial symbiosis with some aquatic ferns
  3. Plant growth stimulation by phosphate-solubilising bacteria
  4. Gall formation on certain angiosperms by Agrobacterium
Ques. 10AIPMT 2004

Restriction endonucleases :-

  1. Are used for in vitro DNA synthesis
  2. Are present in mammalian cells for degradation of DNA when the cell dies
  3. Are synthesized by bacteria as part of their defense mechanism
  4. Are used in genetic engineering for ligating two DNA molecules
Ques. 11AIPMT 2004

In transgenics expression of transgene in target tissue is determined by :-

  1. Enhancer
  2. Promoter
  3. Reporter
  4. Transgene

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