Biotechnology: Principles and Processes

Insertion of a foreign DNA at BamHI site in an E. coli cloning vector pBR322 results in the loss of antibiotic resistance towards :

1. Ampicillin and tetracycline
2. Ampicillin
3. Tetracycline
4. Gentamycin

Match List-I with List-II.

1. A-IV, B-I, C-III, D-II
2. A-II, B-I, C-IV, D-III
3. A-I, B-II, C-IV, D-III
4. A-III, B-IV, C-II, D-I

Given below are two statements: In the light of the above statements, choose the most appropriate answer from the options given below:

1. Statement I is incorrect but Statement II is correct
2. Both Statement I and Statement II are correct
3. Both Statement I and Statement II are incorrect
4. Statement I is correct but Statement II is incorrect

[figure] In the above represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?

1. White color colonies will be selected.
2. Blue color colonies grown on ampicillin plates can be selected.
3. Using ampicillin & tetracycline containing medium plate.
4. Blue color colonies will be selected.

The blue and white selectable markers have been developed which differentiate recombinant colonies from non-recombinant colonies on the basis of their ability to produce colour in the presence of a chromogenic substrate. Given below are two statements about this method: In the light of the above statements, choose the most appropriate answer from the options given below:

1. Statement I is correct but Statement II is incorrect
2. Statement I is incorrect but Statement II is correct
3. Both Statement I and Statement II are correct
4. Both Statement I and Statement II are incorrect

The "Ti plasmid" of Agrobacterium tumefaciens stands for

1. Tumour inhibiting plasmid
2. Tumor independent plasmid
3. Tumor inducing plasmid
4. Temperature independent plasmid

The following diagram shows restriction sites in E. coli cloning vector pBR322. Find the role of 'X' and 'Y' genes : [figure]

1. The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid.
2. The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid.
3. The gene 'X' is for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics.
4. Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance.

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.

1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI

Which of the following is not a cloning vector?

1. YAC
2. pBR322
3. Probe
4. BAC

Which of the following is not a desirable feature of a cloning vector?

1. Presence of origin of replication
2. Presence of a marker gene
3. Presence of single restriction enzyme site
4. Presence of two or more recognition sites

Plasmid pBR322 has Pst I restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactosidase production and the recombinant plasmid is inserted in an E. coli strain

1. It will be able to produce a novel protein with dual ability
2. It will not be able to confer ampicillin resistance to the host cell
3. The transformed cells will have the ability to resist ampicillin as well as produce -galactosidase
4. It will lead to lysis of host cell

The sequence that controls the copy number of the linked DNA in the vector, is termed

1. Recognition site
2. Ori site
3. Selectable marker
4. Palindromic sequence

A gene whose expression helps to identify transformed cell is known as

1. Selectable marker
2. Plasmid
3. Vector
4. Structural gene

Which of the following is not a feature of the plasmids ?

1. Single-stranded
2. Transferable
3. Independent replication
4. Circular structure

The DNA molecule to which the gene of interest is integrated for cloning is called -

1. Transformer
2. Template
3. Vector
4. Carrier

The introduction of T-DNA into plants involves -

1. Altering the pH of the soil, then heat-shocking the plants
2. Infection of the plant by Agrobacterium tumefaciens
3. Exposing the plants to cold for a brief period
4. Allowing the plant roots to stand in water

Which vector can clone only a small fragment of DNA ?

1. Cosmid
2. Bacterial artificial chromosome
3. Yeast artificial chromosome
4. Plasmid

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of :

1. Insertional inactivation of alphagalactosidase in recombinant bacteria
2. Inactivation of glycosidase enzyme in recombinant bacteria
3. Insertional inactivation of alphagalactosidase in non-recombinant bacteria
4. Non-recombinant bacteria containing betagalactosidase

In genetic engineering, the antibiotics are used

1. As sequences from where replication starts
2. As selectable markers
3. To keep the cultures free of infection
4. To select healthy vectors

The figure below is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)? [figure]

1. ori - original restriction enzyme
2. ampR, tetR - antibiotic resistance genes
3. rop - reduced osmotic pressure
4. Hind III, EcoR I - selectable markers

In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector / vectors –

1. B and D only
2. A and C only
3. A, B and D only
4. A only

Which one of the following is used as vector for cloning genes into higher organisms ?

1. Retrovirus
2. Salmonella typhimurium
3. Rhizopus nigricans
4. Baculovirus

Which of the following are used in gene cloning ?

1. Mesosomes
2. Nucleoids
3. Lomasomes
4. Plasmids

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

1. Trichoderma harzianum
2. Meloidogyne incognita
3. Agrobacterium tumefaciens
4. Penicillium expansum

Two microbes found to be very useful in genetic engineering are-

1. Diplococcus sp. and Pseudomonas sp.
2. Vibrio cholerae and a tailed bacteriophage
3. Crown gall bacterium and Caenorhabditis elegans
4. Escherichia coli and Agrobacterium tumefaciens

The most thoroughly studied of the known bacteria-plant interactions is the :-

1. Nodulation of Sesbania stems by nitrogen fixing bacteria
2. Cyanobacterial symbiosis with some aquatic ferns
3. Plant growth stimulation by phosphate-solubilising bacteria
4. Gall formation on certain angiosperms by Agrobacterium

In transgenics expression of transgene in target tissue is determined by :-

1. Enhancer
2. Promoter
3. Reporter
4. Transgene

The Ti plasmid is often used for making transgenic plants. This plasmid is found in

1. Agrobacterium
2. Rhizobium of the roots of leguminous plants
3. Azotobacter
4. Yeast as a 2 plasmid

Which one of the following bacteria has found extensive use in genetic engineering work in plants ?

1. Xanthomonas citri
2. Agrobacterium tumefaciens
3. Bacillus coagulans
4. Clostridium septicum

Maximum number of bases in plasmids discovered so far:

1. 500 kilobases
2. 5 kilobases
3. 50 kilobases
4. 5000 kilobases