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Molecular Basis of Inheritance

AIPMT 2014MCQEasy📈 Topic in 25% papers+4−1
Ques. 1AIPMT 2014

An analysis of chromosomal DNA using the Southern hybridization technique does not use:

  1. Blotting
  2. PCR
  3. Electrophoresis
  4. Autoradiography

Answer

(2) PCR

Solution

As per NCERT, the steps of DNA fingerprinting (Southern blot hybridisation) are:

(i) isolation of DNA, (ii) digestion of DNA by restriction endonucleases, (iii) separation of DNA fragments by electrophoresis, (iv) transferring (blotting) of separated DNA fragments to synthetic membranes such as nitrocellulose or nylon, (v) hybridisation using a labelled VNTR probe, and (vi) detection of hybridised DNA fragments by autoradiography.

∴ Blotting, electrophoresis and autoradiography are used, but PCR is not a step of Southern hybridisation.

Answer: (2)

Keep practising — Molecular Basis of Inheritance

Ques. 2AIPMT 2014

Commonly used vectors for human genome sequencing are :

  1. BAC and YAC
  2. T/A Cloning Vectors
  3. T-DNA
  4. Expression Vectors
Ques. 3NEET 2013

The diagram shows an important concept in the genetic implication of DNA. Fill in the blanks A to C. [figure]

  1. A-translation B-extension C-Rosalind Franklin
  2. A-translation B-transcription C-Erwin Chargaff
  3. A-transcription B-translation C-Francis Crick
  4. A-transcription B-replication C-James Watson
Ques. 4NEET 2013

Which enzyme will be produced in a cell in which there is a non-sense mutation in the lac Y gene ?

  1. Transacetylase
  2. Lactose permease and transacetylase
  3. Lactose permease
  4. -galactosidase
Ques. 5NEET 2013 (Karnataka)

[figure] The figure gives an important concept in the genetic implication of DNA. Fill the blanks A, B and C.

  1. A - Erwin Chargaff B - translation C - replication
  2. A - Maurice Wilkins B - transcription C - translation
  3. A - Francis Crick B - translation C - transcription
  4. A - James Watson B - replication C - extension
Ques. 6NEET 2013 (Karnataka)

Which of the following is not a property of the genetic code ?

  1. Ambiguous
  2. Universal
  3. Degeneracy
  4. Non-overlapping
Ques. 7NEET 2013 (Karnataka)

In an inducible operon, the genes are :

  1. usually expressed unless a signal turns them "off"
  2. always expressed
  3. never expressed
  4. usually not expressed unless a signal turns them "on"
Ques. 8NEET 2013 (Karnataka)

One of the most frequently used techniques in DNA fingerprinting is :-

  1. SSCP
  2. SCAR
  3. VNTR
  4. AFLP
Ques. 9AIPMT 2012 (Mains)

Read the following four statements (A-D) : How many of the above statements are right ?

  1. Four
  2. Two
  3. Three
  4. One
Ques. 10AIPMT 2012 (Mains)

What is it that forms the basis of DNA Fingerprinting ?

  1. The relative proportions of purines and pyrimidines in DNA
  2. The relative amount of DNA in the ridges and grooves of the fingerprints
  3. The relative difference in the DNA occurrence in blood, skin and saliva
  4. Satellite DNA occurring as highly repeated short DNA segments
Ques. 11AIPMT 2012 (Prelims)

Removal of introns and joining of exons in a defined order during transcription is called

  1. Looping
  2. Inducing
  3. Slicing
  4. Splicing

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