Biotechnology: Principles and Processes

Which of the following statements are not true regarding restriction endonucleases? Choose the answer from the options given below :

1. C and D only
2. A and E only
3. D and E only
4. A and B only

Which of the following statements are correct with respect to DNA separation, isolation and visualization? Choose the correct answer from the options given below :

1. A and B only
2. B and D only
3. A and D only
4. B and C only

Which of the following enzyme(s) are NOT essential for gene cloning? Choose the correct answer from the options given below:

1. D and E only
2. B and C only
3. C and D only
4. A and B only

Given below are two statements: In the light of the above statements, choose the most appropriate answer from the options given below :

1. Statement I is correct but statement II is incorrect
2. Statement I is incorrect but statement II is correct
3. Both statement I and statement II are correct
4. Both statement I and statement II are incorrect

Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of:

1. 8 bp
2. 6 bp
3. 4 bp
4. 10 bp

Identify the incorrect statement related to gel electrophoresis.

1. Separated DNA fragments can be directly seen under UV radiation
2. Separated DNA can be extracted from gel piece
3. Fragment of DNA moves toward anode
4. Sieving effect of agarose gel helps in separation of DNA fragments

Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:

1. Hind II, Hind II
2. Hind II, Alu I
3. Hind II, EcoR I
4. Hind II, BamHI

Which of the following are correct about EcoRI? Choose the correct answer from the options given below:

1. B, C, E only
2. A, D, E only
3. A, C, D only
4. B, C, D only

Upon exposure to UV radiation, DNA stained with ethidium bromide will show

1. Bright blue colour
2. Bright yellow colour
3. Bright orange colour
4. Bright red colour

Which one of the following statements is not true regarding gel electrophoresis technique?

1. The process of extraction of separated DNA strands from gel is called elution.
2. The separated DNA fragments are stained by using ethidium bromide.
3. The presence of chromogenic substrate gives blue coloured DNA bands on the gel.
4. Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.

In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?

1. 5'GATACT3' ; 3'CTATGA5'
2. 5'GAATTC3' ; 3'CTTAAG5'
3. 5'CTCAGT3' ; 3'GAGTCA5'
4. 5'GTATTC3' ; 3'CATAAG5'

Given below are two statements: In the light of the above statements, choose the most appropriate answer from the options given below :

1. Both Statement I and Statement II are correct
2. Both Statement I and Statement II are incorrect
3. Statement I is correct but Statement II is incorrect
4. Statement I is incorrect but Statement II is correct

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). In the light of the above statements, choose the correct answer from the options given below :

1. (A) is not correct but (R) is correct
2. Both (A) and (R) are correct and (R) is the correct explanation of (A)
3. Both (A) and (R) are correct but (R) is not the correct explanation of (A)
4. (A) is correct but (R) is not correct

Separation of DNA fragments is done by a technique known as

1. Gel electrophoresis
2. Polymerase Chain Reaction
3. Recombinant technology
4. Southern blotting

Refer to the following statements for agarose-gel electrophoresis: Choose the most appropriate answer from the options given below :

1. B, C and D only
2. A and B only
3. A, B and C only
4. A, B and D only

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as

1. Bright blue bands
2. Yellow bands
3. Bright orange bands
4. Dark red bands

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :

1. Poly (A) tail sequences
2. Degenerate primer sequence
3. Okazaki sequences
4. Palindromic Nucleotide sequences

In gel electrophoresis, separated DNA fragments can be visualized with the help of

1. Ethidium bromide in infrared radiation
2. Ethidium bromide in UV radiation
3. Acetocarmine in bright blue light
4. Acetocarmine in UV radiation

Identify the wrong statement with regard to Restriction Enzymes

1. Sticky ends can be joined by using DNA ligases.
2. They cut the strand of DNA at palindromic sites.
3. Each restriction enzyme functions by inspecting the length of a DNA sequence.
4. They are useful in genetic engineering

The specific palindromic sequence which is recognized by EcoRI is

1. 5′ - GGATCC - 3′ 3′ - CCTAGG - 5′
2. 5′ - GAATTC - 3′ 3′ - CTTAAG - 5′
3. 5′ - GGAACC - 3′ 3′ - CCTTGG - 5′
4. 5′ - CTTAAG - 3′ 3′ - GAATTC - 5′

Choose the correct pair from the following

1. Exonucleases – Make cuts at specific positions within DNA
2. Ligases – Join the two DNA molecules
3. Polymerases – Break the DNA into fragments
4. Nucleases – Separate the two strands of DNA

Following statements describe the characteristics of the enzyme Restriction Endonuclease. Identify the incorrect statement.

1. The enzyme cuts the sugar-phosphate backbone at specific sites on each strand
2. The enzyme recognizes a specific palindromic nucleotide sequence in the DNA
3. The enzyme cuts DNA molecule at identified position within the DNA
4. The enzyme binds DNA at specific sites and cuts only one of the two strands

The DNA fragments separated on an agarose gel can be visualised after staining with

1. Bromophenol blue
2. Aniline blue
3. Ethidium bromide
4. Acetocarmine

DNA fragments are

1. Positively charged
2. Either positively or negatively charged depending on their size
3. Neutral
4. Negatively charged

What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

1. Positively charged fragments move to farther end
2. Negatively charged fragments do not move
3. The smaller the fragment size, the farther it moves
4. The larger the fragment size, the farther it moves

Which of the following is a restriction endonuclease ?

1. DNase I
2. Hind II
3. RNase I
4. Protease

Which of the following restriction enzymes produces blunt ends ?

1. Eco RV
2. Hind III
3. Xho I
4. Sal I

The cutting of DNA at specific locations became possible with the discovery of -

1. Restriction enzymes
2. Selectable markers
3. Probes
4. Ligases

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

1. Polymerase chain reaction
2. Restriction mapping
3. Electrophoresis
4. Centrifugation

Which one of the following represents a palindromic sequence in DNA ?

1. 5′-GAATTC-3′ 3′-CTTAAG-5′
2. 5′-GATACC-3′ 3′-CCTAAG-5′
3. 5′-CCAATG-3′ 3′-GAATCC-5′
4. 5′-CATTAG-3′ 3′-GATAAC-5′