Biotechnology: Principles and Processes

The following diagram shows restriction sites in E. coli cloning vector pBR322. Find the role of 'X' and 'Y' genes : [figure]

1. The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid.
2. The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid.
3. The gene 'X' is for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics.
4. Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance.

In a chromosome, there is a specific DNA sequence, responsible for initiating replication. It is called as:

1. Recognition sequence
2. Cloning site
3. Restriction site
4. ori site

Identify the incorrect statement related to gel electrophoresis.

1. Separated DNA fragments can be directly seen under UV radiation
2. Separated DNA can be extracted from gel piece
3. Fragment of DNA moves toward anode
4. Sieving effect of agarose gel helps in separation of DNA fragments

Recombinant DNA molecule can be created normally by cutting the vector DNA and source DNA respectively with:

1. Hind II, Hind II
2. Hind II, Alu I
3. Hind II, EcoR I
4. Hind II, BamHI

Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.

1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI

Which of the following are correct about EcoRI? Choose the correct answer from the options given below:

1. B, C, E only
2. A, D, E only
3. A, C, D only
4. B, C, D only

Following are the steps involved in the process of PCR. Choose the correct sequence of steps of PCR from the options given below :

1. C A D E B
2. A B E D C
3. A C E D B
4. D B E C A

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out

1. DNA
2. Histones
3. Polysaccharides
4. RNA

In gene gun method used to introduce alien DNA into host cells, microparticles of metal are used.

1. Zinc
2. Tungsten or gold
3. Silver
4. Copper

Upon exposure to UV radiation, DNA stained with ethidium bromide will show

1. Bright blue colour
2. Bright yellow colour
3. Bright orange colour
4. Bright red colour