Biotechnology and its Applications

The Bt toxin in genetically engineered Bt cotton kills the pest by:

1. Creating pores in the midgut
2. Damaging the respiratory system
3. Degenerating the nervous system
4. Altering the pH of body fluids

Following are the steps involved in action of toxin in Bt. Cotton Choose the correct sequence of steps from the options given below:

1. E C B A D
2. B C A E D
3. A E B D C
4. B E C A D

Given below are two statements : In the light of the above statements, choose the correct answer from the options given below.

1. Statement I is true but Statement II is false.
2. Statement I is false but Statement II is true.
3. Both Statement I and Statement II are true.
4. Both Statement I and Statement II are false.

Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment?

1. Serum and Urine analysis
2. Polymerase Chain Reaction (PCR) technique
3. Enzyme Linked Immuno-Sorbent Assay (ELISA) technique
4. Recombinant DNA Technology

Transposons can be used during which one of the following?

1. Polymerase Chain Reaction
2. Gene Silencing
3. Autoradiography
4. Gene sequencing

In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because:

1. Retroviral vector is introduced into these lymphocytes.
2. Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages
3. Lymphocytes from patient's blood are grown in culture, outside the body
4. Genetically engineered lymphocytes are not immortal cells.

Statements related to human Insulin are given below. Which statements is/are correct about genetically engineered Insulin? Choose the most appropriate answer from the options given below :

1. A, B and D only
2. B only
3. C and D only
4. C, D and E only

Milk of transgenic ‘Cow Rosie’ was nutritionally more balanced product for human babies than natural cow milk because it contained:

1. Human enzyme Adenosine Deaminase (ADA)
2. Human protein α-1-antitrypsin
3. Human alpha-lactalbumin
4. Human insulin-like growth factor

When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as :

1. Safety testing
2. Biopiracy
3. Gene therapy
4. Molecular diagnosis

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

1. Detection of gene mutation
2. Molecular diagnosis
3. Gene amplification
4. Purification of isolated protein